Journal: Cell

Article Title: DE NOVO EPIGENETIC PROGRAMS INHIBIT PD-1 BLOCKADE-MEDIATED T-CELL REJUVENATION

doi: 10.1016/j.cell.2017.06.007

Figure Lengend Snippet: Key Resources Table

Article Snippet: Taq polymerase–based PCR kit , QIAGEN , Cat#201225.

Techniques: Virus, Recombinant, DNA Methylation Assay, Plasmid Preparation, Cloning, cDNA Synthesis, Microarray, Software, Methylation Sequencing, Flow Cytometry

Journal: Cancers

Article Title: Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia

doi: 10.3390/cancers13112691

Figure Lengend Snippet: The commercial HPV nucleic acid detection systems used in East, Southeast, and South Asia.

Article Snippet: , , Human Papillomavirus Genotyping Detection Kit (Microarray) (Crystal Core ® , CapitalBio Corporation, Beijing, China) , 22 HPV genotypes: , , , PCR-flow through hybridization fluorescence and gene chip system , [ , ] .

Techniques: Diagnostic Assay, Hybridization, Fluorescence, Quantitative RT-PCR, Microarray, Amplification, Multiplex Assay, Dot Blot, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Polymerase Chain Reaction, Modification, Immunostaining, Next-Generation Sequencing, Biomarker Discovery, Chromatin Immunoprecipitation, SYBR Green Assay, Immunohistochemistry, In Situ Hybridization, Reverse Transcription, Flow Cytometry, Luminex

Journal: Cell cycle (Georgetown, Tex.)

Article Title: DEK Expression is controlled by E2F and deregulated in diverse tumor types.

doi: 10.4161/cc.5.11.2801

Figure Lengend Snippet: Figure 1. E2F1 and E2F3 induce DEK mRNA accumulation. (A) Immunofluorescence analy- sis of U-2 OS-HAERE2F3 inducible cell line.15 U-2 OS-HAERE2F3 were induced with 1µM OHT for 24 hours and stained anti-HA (HA.11, BAbCO). (B) Quantitative analysis of DEK mRNA expression upon induction of U-2 OS-HAERE2F3 with OHT. Q-PCR using SYBR Green reaction mix (Perkin Elmer) was performed on total RNA prepared from U-2 OS-HAERE2F3 cells after 24 hours of induction with OHT and normalized to GAPDH expression. (C, D) DEK expression upon E2F1 and E2F3 induction is independent of pro- tein synthesis. U-2 OS-HAERE2F3 (C) and U-2 OS-HAERE2F130 (D) were treated for 24 hours with or without OHT (1 µM) in the absence or presence of cycloheximide (10µg/ml). Q-PCR on total RNA samples was performed as before.

Article Snippet: The DEK promoter fragment was cloned from human genomic DNA (Roche) using Taq PCR core kit (Qiagen).

Techniques: Immunofluorescence, Staining, Expressing, SYBR Green Assay

Journal: Cell cycle (Georgetown, Tex.)

Article Title: DEK Expression is controlled by E2F and deregulated in diverse tumor types.

doi: 10.4161/cc.5.11.2801

Figure Lengend Snippet: Figure 4. DEK is overexpressed in many human tumors. Tissue microarray (TMA) analysis was performed on 5 TMAs constructed with 460 formalin fixed and paraffin embedded human tumor samples. To detect DEK mRNA expression, in situ hybridization (ISH) was performed with a [35S]UTP-labeled riboprobe designed in the region between nucleotide 380 and 668. (A) DEK is highly expressed in colon and lung primary tumors. The levels of DEK mRNA were deter- mined by ISH on TMA. For each type of tumors normal (N) and tumor (T) counterparts are taken from the TMA. The bright field panels show the tissue sample stained with hematoxylin and eosin counterstaining. The dark field panels show the analysis of DEK expression. (B) Summary table of DEK expression on the TMA tested. (C) DEK expression in Colon, Lung and Melanoma TMAs. Colon: N, normal; I, hyperplastic polyps; A, adenoma; C, carcinoma. Lung: N, normal; D, dysplastic lesion; IS, carcinoma in situ; T, invasive carcinoma. Skin: N, Naevi; T, primary melanoma; M, metastatic melanoma. (D) 4 x 3 contingency table of DEK and Ki67 expression categories. (E) Relative expression level of DEK in samples with low, medium, and strong Ki67 staining. (F) Q-PCR analysis of DEK mRNA in normal (IMR90, MRC5) and tumor (A549, CALU1) lung cell lines. (G) Western Blot analysis of DEK protein expression using anti DEK monoclonal antibody (Becton-Dickinson) was performed on the same cell lines. Ponceau staining is shown as loading control.

Article Snippet: The DEK promoter fragment was cloned from human genomic DNA (Roche) using Taq PCR core kit (Qiagen).

Techniques: Microarray, Construct, Expressing, In Situ Hybridization, Labeling, Staining, In Situ, Western Blot, Control